Composite

Part:BBa_K2467002

Designed by: Alreem Johar   Group: iGEM17_CMUQ   (2017-10-26)
Revision as of 07:51, 1 November 2017 by Ctelmer (Talk | contribs)

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Consensus proU-B0034-mRFP-B0015

This sensor employs a consensus promoter that was designed to incorporate several mutations to increase expression in response to increases in osmolarity. The sensor will induce expression of RFP when osmolarity is high. The sensor can be utilized to measure salt concentrations to determine if the SRB can survive. The promoter can then be used to produce the dispersin enzyme to degrade the biofilm where the SRB accumulate and corrode the pipes.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 833
    Illegal AgeI site found at 945
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None