Composite

Part:BBa_K2467003:Design

Designed by: Alreem Johar   Group: iGEM17_CMUQ   (2017-10-26)
Revision as of 07:28, 1 November 2017 by Ctelmer (Talk | contribs)

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WT proU-RBS-mRFP-B0015


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 857
    Illegal AgeI site found at 969
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sensor will induce expression of RFP when osmolarity is high. The sensor can be utilized to measure salt concentrations to determine if the SRB can survive. The promoter can then be used to produce the dispersin enzyme to degrade the biofilm where the SRB accumulate and corrode the pipes.


Source

Escherichia coli proU operon

References

May G, Faatz E, Lucht JM, Haardt M, Bolliger M, Bremer E. 1989. Characterization of the osmoregulated Escherichia coli proU promoter and identification of ProV as a membrane-associated protein G. Mol Microbiol 3:1521-1531.