Coding

Part:BBa_K2281007

Designed by: Yingkun Wang   Group: iGEM17_CIEI-BJ   (2017-10-26)
Revision as of 05:53, 1 November 2017 by Zhangxuejing (Talk | contribs)


--PdGES--

Introduction

T--CIEI-BJ--part--007.jpg

LOCUS GU136162

SIZE 1755 bp

DEFINITION Phyla dulcis geraniol synthase (GES),we optimized the codon to suitable for E.coli, and avoid several restriction enzyme cutting sites（EcoRⅠ, XbaⅠ, SpeⅠ, PstⅠ，BamHⅠ, HindⅢ, KpnⅠ, NocoⅠ, NdeⅠ, NotⅠ, SalⅠ, XhoⅠ）

PdGES stands for Phyla dulcis geraniol synthase (GES) mRNA which is an enzyme which bolsters the production of geraniol. Through the MVA or DXP pathway, Geranyl Diphosphate (GPP) can be produced. Then PdGES helps convert GPP to geraniol.

T--CIEI-BJ--Background--parts_fig1.jpg

The diagram shows the complete pathway of the production of citronellol.

胶图：

From the diagram above we can see gene GES has been ligated to the vector pB1SC3 properly.

Sources

SOURCE Phyla dulcis 

 ORGANISM  Phyla dulcis
           Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
           Spermatophyta; Magnoliophyta; eudicotyledons; Gunneridae;
           Pentapetalae; asterids; lamiids; Lamiales; Verbenaceae; Lantaneae;
           Phyla.
                                     20171028075605%21Hevea_brasiliensis-source_2.png

From Phyla dulcis which is a species of perennial herb that is native to southern Mexico, the Caribbean (Cuba, Hispaniola, and Puerto Rico), Central America, Colombia, and Venezuela. It is known by several common names, including Aztec sweet herb, bushy lippia, honeyherb, hierba dulce, and tzopelic-xihuitl (Nahuatl). Its buds are also sold as dushi or dulce (sweet in Papiamento and Spanish respectively) buttons.

References

REFERENCE 1 (bases 1 to 1755) 

 AUTHORS   Yang,T., Stoopen,G., Yalpani,N., Vervoort,J., de Vos,R., Voster,A.,
           Verstappen,F.W., Bouwmeester,H.J. and Jongsma,M.A.
 TITLE     Metabolic engineering of geranic acid in maize to achieve fungal
           resistance is compromised by novel glycosylation patterns
 JOURNAL   Metab. Eng. 13 (4), 414-425 (2011)
  PUBMED   21296182

REFERENCE 2 (bases 1 to 1755) 

 AUTHORS   Yang,T., Stoopen,G., Yalpani,N., Voster,A., Vervoort,J., de Vos,R.,
           Verstappen,F., Bouwmeester,H. and Jongsma,M.
 TITLE     Direct Submission
 JOURNAL   Submitted (27-OCT-2009) Entomology, Plant Research International,
           Droevendaalsesteeg, Wageningen 6700AP, The Netherlands

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 184
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1093


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Parameters
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