Composite

Part:BBa_K2384006

Designed by: Haiyang Chen   Group: iGEM17_FAFU-CHINA   (2017-10-21)
Revision as of 05:38, 1 November 2017 by Freak9631 (Talk | contribs)


Pveg+RBS+CspC+6*HIS+T1 terminator+T7 terminator


This is an improve part.This gene is a cold resistant gene from the Arctic cryophile Polaribacter irgensii.It can largely increase the cold resistance of bacterias.CDS sequence optimization in (BBa_K538004) is better suited for expression in Bacillus megaterium and we add a 6*His tag at the C end.After that, the promoter (BBa_K143012) and RBS (BBa_K090505) were combined.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//awards/basic_part
//function/regulation/transcriptional
Parameters
chassisBacillus megaterium
familyCold shock proteins