Part:BBa_K2272020

pBAD-HAO-Cyt

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Usage and Biology

Figure 1. This graph compares the efficacy of performing nitrification of our model organisms, Paracoccus Denitrificans and Nitrosomonas Europaea, versus our transformed chassis organism, Paracoccus Denitrificans with the HAO-CYT circuit. Efficacy was measured in change in the concentrations of ammonia over time, which showed efficiency of Nitrification in our Bioreactor.

Figure 2. This graph compares the efficacy of producing nitrates, an end product of the nitrification process, between our model organisms, Paracoccus Denitrificans and Nitrosomonas Europaea, versus our transformed chassis organism, Paracoccus Denitrificans with the HAO-CYT circuit.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 1144
  Illegal BamHI site found at 1556
  Illegal XhoI site found at 3120
  Illegal XhoI site found at 5329
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 1572
  Illegal NgoMIV site found at 2049
  Illegal NgoMIV site found at 2053
  Illegal NgoMIV site found at 3821
  Illegal NgoMIV site found at 4211
  Illegal NgoMIV site found at 4478
  Illegal NgoMIV site found at 4526
  Illegal AgeI site found at 979
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 1476
  Illegal BsaI.rc site found at 1524
  Illegal BsaI.rc site found at 2091
  Illegal BsaI.rc site found at 4251
  Illegal BsaI.rc site found at 5928
  Illegal BsaI.rc site found at 6084
  Illegal SapI site found at 961