Coding

Part:BBa_K2194001:Design

Designed by: Catherine Dunaway, Anna Guseva   Group: iGEM17_Rice   (2017-10-23)
Revision as of 02:41, 1 November 2017 by Catdunaway (Talk | contribs) (→‎Source)


Sulfate Binding Protein (sbp) cds


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 3
    Illegal BsaI.rc site found at 1006


Design Notes

Primers used to PCR amplify sbp from the Escherichia coli MG1655 genomeNOTE:

5' CGATCGTCTCACTCGAATGAACAAGTGGGGCGTAG3'

5' GCATCGTCTCACTCTGCCATCAGCGTTTGCTGATCTG3'


NOTE: The primer overhangs are bolded.

Source

We sourced the nucleotide sequence for sbp from the Escherichia coli MG1655 genome EcoCyc database (Accession ID EG10929). We used this sequence to design primers to PCR amplify the sequence from purified Escherichia coli MG1655 genome.

References