Coding

Part:BBa_K2194001:Design

Designed by: Catherine Dunaway, Anna Guseva   Group: iGEM17_Rice   (2017-10-23)
Revision as of 02:40, 1 November 2017 by Catdunaway (Talk | contribs) (→‎Design Notes)


Sulfate Binding Protein (sbp) cds


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 3
    Illegal BsaI.rc site found at 1006


Design Notes

Primers used to PCR amplify sbp from the Escherichia coli MG1655 genomeNOTE:

5' CGATCGTCTCACTCGAATGAACAAGTGGGGCGTAG3'

5' GCATCGTCTCACTCTGCCATCAGCGTTTGCTGATCTG3'


NOTE: The primer overhangs are bolded.

Source

Escherichia coli genomic sequence

References