Translational_Unit
EutSMN

Part:BBa_K2213012

Designed by: Adam Hannaford   Group: iGEM17_Manchester   (2017-10-10)
Revision as of 02:20, 1 November 2017 by AmberJam (Talk | contribs)


LacUV5_EutS_Tet_EutMN

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Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1260
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 4125
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 3451


Function:
This part was expressed alongside EutLK-Low-PduD-mCherry-PPK (https://parts.igem.org/Part:BBa_K2213013) to form EutSMNLK+Low_PduD+PPK which was characterized as follows.

A 24 hour induction was DAPI stained to determine microcompartment formation and tag localisation.

LowSMNLKDAPIcells.png
Figure 2. Fluorescence microscopy images of promoter-PduD associated mCherry and DAPI stained polyphosphate.

As shown, there is a heterogeneous distribution of mCherry signal indicating successful tag localisation and by proxy successful expression of Eut subunits.

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Categories
Parameters
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