Part:BBa_K2272001

promoter MnSOD

A promoter that regulates the production of MnSOD (mitochondrial antioxidant manganese superoxide dismutase), which detoxify radical oxygen produced by mitochondrial respiration. Under oxidative stress or hypoxic modulation, MnSOD will be upregulated by an increase of its promoter activity as well as by other complex transcriptional/post-transcriptional modifications.

Usage and Biology

Read-out fluoresence level of GFP from the reporter circuit of pMnSOD comparing to dissolved oxygen level and cell density.

As shown in the Figure above, the decrease in dissolved oxygen level lead to the upregulation of GFP repression, which suggested the promoter MnSOD was being induced under minimally aerobic condition. The experiment was conducted in a way that utilized parafilm to seal the culture tube and create oxidative stress. Replicates of culture tubes were simultaneously incubated and sealed at different time points, so that sets of the same cell culture under different aerobic conditions could be compared and tested for fluoresence expression level.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]