DNA

Part:BBa_K2440035

Designed by: Qijie Xu   Group: iGEM17_NUDT_CHINA   (2017-10-22)
Revision as of 01:27, 1 November 2017 by MINXIAO (Talk | contribs) (→‎Experimental Validation)

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miR7491 target sequence

It is the target sequence of miR7491, a modularized DNA part from a set of chemically synthetic oligo DNA library.

Usage and Biology

MiRNA locker assembled by using this modularized DNA part was able to bind miR7491 in an Ago2 dependent manner, that is, knockdown of miR7491 was achieved by transfecting cells with miRNA locker.

Ghr-miR7491 was firstly found in cotton ovules1. And its function is still unknown.

Sequence and Features

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Experimental Validation

This part is sequenced as correct after construction.


Reference

1.A comparative miRNAome analysis reveals seven fiber initiation-related and 36 novel miRNAs in developing cotton ovules.Wang ZM, Xue W, Dong CJ, Jin LG, Bian SM, Wang C, Wu XY, Liu JY Mol Plant. 5:889-900(2012).

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