Part:BBa_K2440032

miR-489 target sequence

It is the target sequence of miR-489, a modularized DNA part from a set of chemically synthetic oligo DNA library.

Usage and Biology

MiRNA locker assembled by using this modularized DNA part was able to bind miR-489 in an Ago2 dependent manner, that is, knockdown of miR-489 was achieved by transfecting cells with miRNA locker.

MiR-489 was firstly found in the adult fish’s brain and eye.¹

Compared to the corresponding non-tumor tissues, miR-489 was frequently downregulated in colorectal cancer (CRC). By Kaplan-Meier analysis, lower CRC recurrence free survival years in the group with elevated miR-489 expression than those with lower miR-489 expression. Moreover, miR-489 targets the 3'-UTR of the HDAC7 transcript and downregulates its expression, and HDAC7 expression promoted tumor cell proliferation and invasion. In conclusion, miR-489 suppresses tumor invasion and metastasis in CRC by targeting HDAC7.¹

Moreover, miR-489 can inhibit proliferation, cell cycle progression and induces apoptosis of glioma cells via targeting SPIN1-mediated PI3K/AKT pathway.¹

Sequence and Features

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Experimental Validation

This part is sequenced as correct after construction.