DNA

Part:BBa_K2440027

Designed by: Qijie Xu   Group: iGEM17_NUDT_CHINA   (2017-10-22)
Revision as of 01:13, 1 November 2017 by MINXIAO (Talk | contribs) (→‎Experimental Validation)

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miR397b target sequence

It is the target sequence of miR397b, a modularized DNA part from a set of chemically synthetic oligo DNA library.

Usage and Biology

MiRNA locker assembled by using this modularized DNA part was able to bind miR397b in an Ago2 dependent manner, that is, knockdown of miR397b was achieved by transfecting cells with miRNA locker.

Aly-miR397b-5p is a novel miRNA that was firstly found in Arabidopsis lyrata1. And its function is still unknown.

Sequence and Features

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Experimental Validation

This part is sequenced as correct after construction.


Reference

1.Arabidopsis lyrata small RNAs: transient MIRNA and small interfering RNA loci within the Arabidopsis genus. Ma Z, Coruh C, Axtell MJ Plant Cell. 22:1090-1103(2010).

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