DNA
Part:BBa_K2440024
Designed by: Qijie Xu Group: iGEM17_NUDT_CHINA (2017-10-22)
miR817 target sequence
It is the target sequence of miR817, a modularized DNA part from a set of chemically synthetic oligo DNA library.
Usage and Biology
MiRNA locker assembled by using this modularized DNA part was able to bind miR817 in an Ago2 dependent manner, that is, knockdown of miR817 was achieved by transfecting cells with miRNA locker.
MiR817 was firstly found in Oryza sativa and its target positions are:Os02g12800(0) Os03g31180(0) Os02g07960(1.5) Os09g39910(2)Os03g29680(0),Os08g38620(2).Its predicted functions are related to Elongation factor, Diacylglycerol kinase, and ABC transporter.1
Sequence and Features
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Experimental Validation
This part is sequenced as correct after construction.
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Categories
Parameters
None |