DNA

Part:BBa_K2440022

Designed by: Qijie Xu   Group: iGEM17_NUDT_CHINA   (2017-10-22)
Revision as of 01:05, 1 November 2017 by MINXIAO (Talk | contribs) (→‎Experimental Validation)

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miR418 target sequence

It is the target sequence of miR418, a modularized DNA part from a set of chemically synthetic oligo DNA library.

Usage and Biology

MiRNA locker assembled by using this modularized DNA part was able to bind miR418 in an Ago2 dependent manner, that is, knockdown of miR418 was achieved by transfecting cells with miRNA locker.

Osa-miR418 was firstly found in Arabidopsis. It is located in Chr3, ds of at 3g18890(a) and we still does not find its homolog of known Arabidopsis miRNAs.1

Sequence and Features

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Experimental Validation

This part is sequenced as correct after construction.


Reference

1.Prediction and identification of Arabidopsis thaliana microRNAs and their mRNA targets. Wang XJ, Reyes JL, Chua NH, Gaasterland T Genome Biol. 5:R65(2004).

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