DNA

Part:BBa_K2440013

Designed by: Qijie Xu   Group: iGEM17_NUDT_CHINA   (2017-10-22)
Revision as of 00:39, 1 November 2017 by MINXIAO (Talk | contribs) (Usage and Biology)


miR-16 target sequence

It is the target sequence of miR-16, a modularized DNA part from a set of chemically synthetic oligo DNA library.

Usage and Biology

MiRNA locker assembled by using this modularized DNA part was able to bind miR-16 in an Ago2 dependent manner, that is, knockdown of miR-16 was achieved by transfecting cells with miRNA locker.

MiR-16 was firstly detected in mouse kidney, adult zebrafish, and very weakly in frog ovary1. Located at chromosome 13q12, it is encoded by the mir-15a/mir16-1 and the mir-15b/mir16-2polycistrons.And its relative cloning frequency varied greatly and was higher in tumor samples and cell lines compared to normal tissues3.

Reductions in expression of miR-15a and miR-16, let-7a, or miR-143 and miR-145 have been reported in chronic lymphocytic leukemia , lung cancer , and colorectal neoplasia , respectively4.

Sequence and Features

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Experimental Validation

This part is sequenced as correct after construction.

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Categories
Parameters
None