Translational_Unit

Part:BBa_K2213004

Designed by: Jessica Burns   Group: iGEM17_Manchester   (2017-08-17)
Revision as of 00:33, 1 November 2017 by Jengleback (Talk | contribs)


PduD(1-20)_cgPPK2_mCherry_His6

-

Usage and Biology


This part is a fusion of the PduD(1-20), cgPPK and mCherry followed by a hexahistidine tag. We intended the PduD(1-20) sequence to localise the cgPPK into a recombinant microcompartment and the mCherry would have allowed us to confirm its co-localisation in the microcompartment.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 148
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1030
    Illegal SapI.rc site found at 827


[edit]
Categories
Parameters
None