Composite

Part:BBa_K2443022

Designed by: Lethbridge iGEM 2017   Group: iGEM17_Lethbridge   (2017-10-18)
Revision as of 23:58, 31 October 2017 by Harlando (Talk | contribs)


MTF optimized for expression in E. coli

Methionyl-tRNA transformyalse (MTF) is a zinc finger metal response element-binding transcription factor. Codon optimized for use in Escherichia coli. Contains a C-terminal hexahistidine tag.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 888
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 244
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//collections/cell-free_tx-tl
Parameters
None