Composite

Part:BBa_K2109108

Designed by: Andy Hudson   Group: iGEM16_Lethbridge   (2016-10-14)
Revision as of 23:36, 31 October 2017 by Harlando (Talk | contribs)


MS2 6XHis (V29I/d1FG)

this part is an improvement upon BBA_K1791003 from the Lethbridge iGEM 2015 submission. It contains a N terminal Hexahistidine tag on a modified MS2 coat protein sequence. The sequence has been codon optimized for Ecoli and modified via a V29I mutation and a 13 aa deletion with a Gly/Ala insertion. These mutations prevent capsid formation as well as dimerization of MS2 for more efficient yields during purification.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 248
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//collections/cell-free_tx-tl
Parameters
None