Part:BBa_K2377006
TrxzN, a developed element to generate trxz hpRNA with a intron loop
As the intron sequence will be sliced during the pre-mRNA processing and the stability is much higher than dsRNA and hpRNA with a normal loop according to a relevant paper, it will be a better option for us to silence the target sequene. So we constructed this composite part to generate trxz hpRNA with a intron loop.
TRX z is a branch of plastidial thioredoxin (TRX), lying between x- and y-type TRXs thus was named TRX z, which is a likely candidate for mediating thiol-modifications in chloroplasts.
Recently, several independent studies could demonstrate that TRX z constitutes a subunit of the plastid-encoded RNA polymerase (PEP). Meanwhile the generation of the full PEP complement is an essential early step during chloroplast bio-genesis and its disturbance cannot be reversed if a certain time point in the program has been passed.
The Arabidopsis knockout mutant lines of TRX z exhibit a complete albino phenotype and was inhibited in chloroplast development.What’s more, we noticed that a analysis of promoter-GUS indicated that TRX z is expressed in young tissues with decreasing expression during leaf maturation. So our verification need to be at the young stage of the arabidopsis otherwise the phenotype
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 154
Illegal AgeI site found at 1144 - 1000COMPATIBLE WITH RFC[1000]
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