Part:BBa_K2377006
TrxzN, a developed element to generate trxz hpRNA with a intron loop
As the intron sequence will be sliced during the pre-mRNA processing and the stability is much higher than dsRNA and hpRNA with a normal loop according to a relevant paper, it will be a better option for us to silence the target sequene.
The ecdysone receptor (ECR) is a such kind of gene, which could up-regulate the vital genes expression through the interaction with ultra-stable protein (USP) under the existence of ecdysteroid hormone. Therefore, Ecr gene silencing can effectively increasing the insects mortality and reducing the reproduction rate. Studies have shown when fed aphids with 7.5 ng / ul dsRNA Ecr, the mortality rate up to 70% after 8 days. According to the literature, the similarity of Ecr gene sequence between pea aphid and grain aphid was 98.4% and with more than 90% similarity in other kind of aphids, so Ecr is an Ideal RNAi target site to kill aphids with a broad spectrum destruction effect.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 154
Illegal AgeI site found at 1144 - 1000COMPATIBLE WITH RFC[1000]
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