Part:BBa_K2271062

pex13-mTurqoise

Usage and Biology

This part is a composite part comtaining the peroxisomal membrane anchor Pex13 with an N-terminal fusion of the CFP variant mTurquoise. It is applicable for S. cerevisiae using the HHF1 promoter and the TDH1 terminator. The optimal excitation is at a wavelength of 434 nm with an excitation optimum at 474 nm.

Experimental Design an Results

The part was used to transform S. cerevisiae BY4742. Transformants were used to validate the part by microscopy using the Elyra PS microscope. The microscope images indicating an localisation of mTurquoise-Pex13 in the peroxisomal membrane (Figure 1).

Figure 1The micrscopy showing an localisation of mTurquoise with the typical peroxisomal shape. This indicates, an peroxisomal membrane licalisation of mTurquoise-Pex13

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal suffix found in sequence at 1987
  Illegal BglII site found at 880
  Illegal BglII site found at 917
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

References

[1] A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly (2015)
Michael E. Lee, William C. DeLoache, Bernardo Cervantes, and John E. Dueber
ACS Synth. Biol., 2015, 4 (9), pp 975–986 DOI: 10.1021/sb500366v