Coding
Part:BBa_K2455003
Designed by: Jon Fugl Group: iGEM17_UCopenhagen (2017-10-27)
Cell-Penetrating USER Cassette
This biobrick contains an AsiSI/Nb.BSMI USER Cassette with a N-terminal nona-histine (R9) Cell-penetrating peptide which gives proteins inserted into the cassette the ability to pass through plasma membranes. In addition the USER Cassette carries a N-terminal hexa-histidine tag which allows for easy purification.
For our study we demonstrated that proteins inserted into the cassette can be purified, using the histidine-tag, and subsequently transferred into E. coli cells through the use of the R9 peptide.
In addition; previous studies have shown proteins associated with R9, either covalently or non-covalently, to be able to enter a variety of cell types (link!).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
//cds/membrane/transporter
//chassis/prokaryote/ecoli
//chassis/prokaryote/ecoli
direction | Forward |
function | Cell-Penetrating, USER Cassette |
tag | C-terminal hexa-histidine |