RNA

Part:BBa_K2340011

Designed by: Laurens Heling   Group: iGEM17_Kent   (2017-10-31)
Revision as of 18:46, 31 October 2017 by LWHeling (Talk | contribs)

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Guide RNA construct of human Inpp1 (3)

Inpp1 guide RNA construct with a human U6 promoter and human U6 terminator.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 217
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 183
    Illegal XhoI site found at 224
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Usage and Biology

This product will be translated by RNA polymerase III to form a guide RNA. This small RNA strand can be processed by the CRISPR dCas13a from Leptotrichia buccalis (LbuC2c2; BBa_K2340000) to form a CRISPR RNA (crRNA).

The sequence that is complementary to Inpp1 is TCAAAAGAGGAAGGTGAATG. This is one of three Inpp1 guide RNA constructs we have constructed


DNA for this part has not been submitted as a sample to the registry due to time restraints.


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Categories
Parameters
None