Reporter

Part:BBa_K1875003

Designed by: Jeffrey Marano   Group: iGEM16_BostonU   (2016-10-11)
Revision as of 17:32, 31 October 2017 by TongYou (Talk | contribs)


This part contains a GFP reporter

This GFP is used in all of the following plasmids: BBa_K1875013, BBa_K1875014, BBa_K1875015, BBa_K1875016, BBa_K1875017, BBa_K1875018, BBa_K1875019.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


CONTRIBUTION

[http://2017.igem.org/Team:Kyoto iGEM Kyoto 2017] improved this part by connecting it with BBa_K530008 (TDH3 promoter) , adding further functions. In this improvement, we obtained a new part BBa_K2403003 .

Combination with BBa_K530008 (TDH3 promoter)

This combination gave this part the function as a reporter in S. cerevisiae. Furthermore, by culturing pine wood nematodes with yeast expressing EGFP, feeding on yeast by pine wood nematodes observed, demonstrating an innovative new use and functional contribution of the original parts.

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Categories
Parameters
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