Coding

Part:BBa_K2336034:Design

Designed by: Ziyang Xiao   Group: iGEM17_HUST-China   (2017-10-14)
Revision as of 17:29, 31 October 2017 by Xzy1012 (Talk | contribs) (→‎Source)

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oprF-GS-FLAG-LBT12-GS-LBT12-GS-LBT12


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 884
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 317
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 953
    Illegal AgeI site found at 5
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 121


Design Notes

We amplificated the oprF and FLAG-LBT separately as the amount of gene synthesized by IDT is too little.Then,we linked these two genes and got the plasmid with these two genes.

Source

Synthesized by IDT.And we fused 2 sequences(oprF and 3*LBT).

References