Part:BBa_K2273107
Evaluation Vector with PxylA to screen for protein specific secretion efficiency
The Evaluation Vector (EV) is part of both, the [http://2017.igem.org/Team:TU_Dresden/Composite_Part Evaluation Vector] and the [http://2017.igem.org/Team:TU_Dresden/Composite_Part Signal Peptide Toolbox] of the [http://2017.igem.org/Team:TU_Dresden iGEM Team TU Dresden 2017 (EncaBcillus - It's a trap!)].
The EV was developed with easily exchangeable units: I) allowing the replacement of the promoter (which drives the system) and II) a multiple cloning site enabling to work with translationally fused composite parts.
In summary, the EV was designed to fulfill the following distinct features:
- Exchangeable promoter region
- Insertion of basic or composite parts as expression units
- Fulfilling the RFC10 and RFC25 BioBrick standard
- Easy cloning and screening procedure in Escherichia coli
In the case of the Signal Peptide Toolbox which was designed to increase protein secretion efficiency, a typical composite part consists of a signal peptide (for secretion in bacteria) and a protein of interest.
Sequence and Features
The DNA sequence of this part has been verified via sequencing before it was sent in.
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 247
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1354
Illegal AgeI site found at 1348 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 266
None |