Reporter

Part:BBa_K895006

Designed by: Frank Sargent   Group: iGEM12_Dundee   (2012-09-23)
Revision as of 16:32, 31 October 2017 by GBY15 (Talk | contribs) (→‎Contribution)


GFP open reading frame

This is GFP from Biobrick BBa_J54103, which we have sequenced and re-cloned to RFC[10] standard in pSB1C3.


Contribution

Group: Team Tsinghua 2017

Author: Boyang Gao

Summary: iGEM 2017 Team Tsinghua has made a significant improvement of this part, by validating that this GFP protein works well in the S. cerevisiae (baking yeast) chassis, and adding an yeast inducible promoter pGalS (BBa_K2247000) in front of this part. The new part we constructed is BBa_K2247001. By making this improvement, we achieved inducible expression of GFP by galactose or by yeast Gal4 protein in yeast cells, and this new composite part can also serve to validate and characterize the function of the pGalS promoter. In future projects applying the yeast-two-hybrid or yeast-one-hybrid system, this new part can serve as a basic and standard reporter plasmid. For experimental validation of the new part, please see the main page of BBa_K2247001.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 644


[edit]
Categories
Parameters
None