Part:BBa_K2336035

oprF-GS-Sitag-FLAG

The report part of our circuit. It can be expressed to bind silicon

Usage and Biology

oprF is motif cell wall anchor domain protein. GS stands for GS linker. Sitag-FLAG is designed to express specially protein. The genetic engineering bacteria can produce the protein on its membrane. Then the bacteria can be absorbed by silica surface. So we can capture them. Figure 1 shows the circuit of it.

Figure 1: The circuit of oprF-GS-Sitag-FLAG.

As we have said, this part is a final part. After sensing and capture finish, the bacteria will be bound onto silica surface. We can achieve our goal to get them(with lanthanum). Figure 2 shows how it works.

Figure 2: The preview of Sitag displayed on cell wall and work.

Characterization

We did some experiment to prove it works.

SDS-PAGE

After oprF-GS-Sitag-FLAG is expressed successfully,we centrifuged the bacteria liquid and separated different proteins by SDS-PAGE. As figure 3 shows.

Figure 2: The preview of Sitag displayed on cell wall and work.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 72
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 639
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 3
  Illegal AgeI site found at 1527
  
• 1000
  COMPATIBLE WITH RFC[1000]