RNA

Part:BBa_K2340001

Designed by: Laurens Heling   Group: iGEM17_Kent   (2017-10-31)
Revision as of 15:54, 31 October 2017 by LWHeling (Talk | contribs)


Guide RNA construct of human ꞵ-actin (1)



Usage and Biology

Human ꞵ-actin guide RNA construct with a human U6 promoter and human U6 terminator. This product will be translated by RNA polymerase III to form a guide RNA. This small RNA strand can be processed by the CRISPR dCas13a from Leptotrichia buccalis (LbuC2c2; BBa_K2340000) to form a CRISPR RNA (crRNA).

The sequence that is complementary to Human ꞵ-actin is AAGTGTTGTTGAAGTTGTCC. This is one of three Human ꞵ-actin guide RNA constructs we have constructed


DNA for this part has not been submitted as a sample to the registry due to time restraints.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 217
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 183
    Illegal XhoI site found at 224
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None