Coding

Part:BBa_K2365035

Designed by: yuanqi jia   Group: iGEM17_NAU-CHINA   (2017-10-14)
Revision as of 15:23, 31 October 2017 by 216 (Talk | contribs) (→‎PCR and SDS-PAGE)


ZHD101--Zearalenone degrading enzyme (optimized)

the gene encoding ZHD101（a ZEN-degrading enzyme）was cloned. ZHD101, a lactonohydrolase produced by the fungal species Clonostachys rosea, converts ZEN into 1-(3,5-dihydroxy-phenyl)-10-hydroxy-1-undecen- 6-one, which is a markedly less toxic product.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


PCR and SDS-PAGE

Showing the size of the target gene and the protein.

ZHD101 DNA.jpg ZHD101 SDS.jpg

[edit]
Categories
Parameters
None