Coding

Part:BBa_K2336035

Designed by: Ziyang Xiao   Group: iGEM17_HUST-China   (2017-10-14)
Revision as of 15:21, 31 October 2017 by Xzy1012 (Talk | contribs)


oprF-GS-Sitag-FLAG

The report part of our circuit. It can be expressed to bind silicon

Usage and Biology

oprF is motif cell wall anchor domain protein. GS stands for GS linker. Sitag-FLAG is designed to express specially protein. The genetic engineering bacteria can produce the protein on its membrane. Then the bacteria can be absorbed by silica surface. So we can capture them. Figure 1 shows the circuit of it.

Figure 1: The circuit of oprF-GS-Sitag-FLAG.

As we have said, this part is

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 72
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 639
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 3
    Illegal AgeI site found at 1527
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None