Regulatory

Part:BBa_K2365040

Designed by: yuanqi jia   Group: iGEM17_NAU-CHINA   (2017-10-14)
Revision as of 14:45, 31 October 2017 by 1314 (Talk | contribs)


PGK1 promoter

PGK1 Yeast promoter Constitutive expression

Phosphoglycerate kinase, phosphoglycerate kinase promoter, transcription start key enzyme of glycolysis, and each species to enzyme must survive, the deficiency of the enzyme can cause organism metabolism disorder, as a strong promoter in gene engineering

Artificial promoter from mature yeast constitutively expressed plasmid amplified promoter, even on the expression vector prs423 after insertion of test elements GFP - adh1t fusion fragment into yeast sey6210 test 475-530nm fluorescence intensity.we test TDH3 PGK1 2HXT7

Ap.png

File:Ap.png


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None