Regulatory
PrapA

Part:BBa_K2273001:Design

Designed by: Jonathan Hammer   Group: iGEM17_TU_Dresden   (2017-09-25)
Revision as of 14:16, 31 October 2017 by YamYam (Talk | contribs)

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PrapA: a ComA-dependent promoter of Bacillus subtilis


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design

This part was generated with RFC10 Suffix and Prefix. It encodes for the rapA promoter that is part of the ComX quorum response of B. subtilis and was designed for the subproject "Communication" by the [http://2017.igem.org/Team:TU_Dresden/Project/Communication iGEM Team TU Dresden 2017 (EncaBcillus - It's a trap!)].

Source

The rapA promoter was amplified via PCR from the B. subtilis wild type W168 genome using the primers listed below.

PrapA fwd RFC10 gatcGAATTCGCGGCCGCTTCTAGAGTTTGTTGCGGTTAGCCGAAATTC
PrapA rev RFC10 gatcCTGCAGCGGCCGCTACTAGTATTTGAATTACCCGAGATATGTC

References

Wolf D, Rippa V, Mobarec JC, Sauer P, Adlung L, Kolb P, Bischofs IB(2016) The quorum-sensing regulator ComA from Bacillus subtilis activates transcription using topologically distinct DNA motifs. Nucleic Acids Res.44(5):2160-72. PubMed

Hamoen LW, Venema G, Kuipers OP (2003) Controlling competence in Bacillus subtilis: shared use of regulators. Microbiology. 149(Pt 1):9-17 PubMed