Generator
Plac-sam8

Part:BBa_I742144

Designed by: Chris French   Group: iGEM07_Edinburgh   (2007-10-23)
Revision as of 18:33, 25 October 2007 by Cfrench (Talk | contribs)

Plac+lacZ+sam8 (tyrosine ammonia lyase)

This part consists of sam8, encoding tyrosine ammonia lyase (converts tyrosine to p-coumaric acid) from the bacterium Saccharothrix espanaensis DSM 44229, inserted downstream of a lac promoter with attached lacZ' minigene. The purpose of this was to ensure sam8 expression on induction with IPTG and also to provide a visually selectable tag when assembling the entire pathway; by always inserting a lacZ-containing part into a non-lacZ-containing part it is easy to select the correct clones on the basis that they form blue colonies.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1416
    Illegal BamHI site found at 1818
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1159
    Illegal AgeI site found at 1106
    Illegal AgeI site found at 1275
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 710
    Illegal BsaI site found at 976


[edit]
Categories
//classic/generator/PRC
Parameters
None