Terminator

Part:BBa_K2110000

Designed by: Haoran Ding   Group: iGEM16_Tianjin   (2016-10-04)
Revision as of 14:08, 31 October 2017 by Eirikbsu (Talk | contribs)


Terminator.png

chassis Saccharomyces cerevisiae, Schizosaccharmoyces Pombe

CYC1 Terminator

  • CYC1 Terminator is widely used in Saccharomyces cerevisiae. It is a composite part in many vectors of Saccharomyces cerevisiae like plasmid pYES2. Amplifying it by PCR and link it after your protein coding sequence is also feasible if you want to express the gene in Saccharomyces cerevisiae.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Team UiOslo Norway 2017 improved the documentation of this part by cloning it into pSB1C3 and sequencing it.

Results from PCR amplification of CYC1 inserted into pSB1C3:

<img src="T--UiOslo_norway--fragmnt_cyc.jpg" width="778" height="880">


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//terminator
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chassis Saccharomyces cerevisiae, Schizosaccharmoyces Pombe