Composite

Part:BBa_K2365052

Designed by: Tong Cheng   Group: iGEM17_NAU-CHINA   (2017-10-18)
Revision as of 14:02, 31 October 2017 by 1314 (Talk | contribs)


TPI1 promotor-CYC1 terminator

Between the TPI1 promoter and CYC1 terminator,having the restriction enzyme cutting site.And you can insert the gene if you want.And it is inserted into the expression vector pRS423 and inserted into GFP (S65T), then transformed into Saccharomyces cerevisiae SEY6210 to test the fluorescence intensity of 440-550nm

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 246
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 318


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Categories
Parameters
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