DNA

Part:BBa_I751400:Design

Designed by: Kenichiro Iwasaki   Group: iGEM07_Tokyo_Tech   (2007-10-25)
Revision as of 18:27, 25 October 2007 by Iwasaki (Talk | contribs) (→‎Design Notes)

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BioBrick to Tokyo Standard adapter, for XbaI, forward


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 20
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

1. Cut Biobrick part with XbaI.

2. Incorporate adapter oligo. (don't forget to anneal with rev, and to make kination)

3. Now you can cut out this part by SalI or BamHI.


This makes Biobrick scar-like sequence, "tctagt" in the left and "actaga" in the right. Once oligo is ligated, you can't cut with XbaI anymore.

Source

References