Composite

Part:BBa_K2371012

Designed by: Wang Xiao   Group: iGEM17_BGIC-Union   (2017-10-24)
Revision as of 13:44, 31 October 2017 by Joyce324 (Talk | contribs)


pT7-amilGFP

This chromoprotein eforRed naturally exhibits red/pink color when expressed. The color is slightly weaker than RFP. On agar plates and in liquid culture, the color is readily visible to naked eye in less than 24 hours of incubation. The DNA was codon-optimized for expression in E.coli and synthesized by the Korean company Bioneer Corporation.

Figure1.PCR test of eforRed and GFP.

Pcr test of eforRed and GFP.png

Figure2.RD test of eforRed and GFP.

RD test of amliRed and GFP.png Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 600
    Illegal AgeI site found at 712
  • 1000
    COMPATIBLE WITH RFC[1000]


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