Coding

Part:BBa_K2365037

Designed by: Wu Yaxuan   Group: iGEM17_NAU-CHINA   (2017-10-14)
Revision as of 11:49, 31 October 2017 by Vickrystal (Talk | contribs)


Trichothecene 3-O-acetyltransferase(AYT1)，an indispensable enzyme for the biosynthesis of trichothecenes, to catalyzes the transfer of an acetyl group from acetyl coenzyme A to the C3 hydroxyl moiety which will result in a switch from DON to 3ADON, so the toxicity will be reduced by 100-fold.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 213
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 306
    Illegal BglII site found at 318
    Illegal XhoI site found at 884
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

PCR and SDS-PAGE

Showing the size of the target gene.

Don degrading enzyme 1.jpg DON degrading enzyme SDS.jpg


Fig3 shows that we use the MSD to analyze the degrading effects of our DON degrading enzyme
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Parameters
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