Part:BBa_K2387054
mRFP controlled by inducible araC/pBAD promoter
mRFP is a red monomeric fluorescent protein. In this composite, its expression is regulated by the araC/pBad promoter and by a strong RBS. mRFP presents a maximum pick of excitation at 584 nm and a maximum pick of emission at 607 nm. mRFP was engineered from DsRed to grant it a monomeric conformation.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1205
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1144
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 979
Illegal AgeI site found at 1793
Illegal AgeI site found at 1905 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 961
Functional Parameters
In order to test the functionality of this protein under the araC/pBad promoter, the absorbance and the fluorescence spectra were measured after induction with 0.02% arabinose (Figure 1).
This protein is part of a collection of split proteins developed for BiFC analysis and tested with the CpxR system (BBa_K2387032). These proteins were characterized through multiple experiments using as interacting proteins antiparallel synthetic leucine zippers. The proteins of this collection are the ones found in Table 1.
Table 1: Split Proteins. | ||
---|---|---|
Protein | Part Number (Full length) | Part Number (Split Protein) |
mRFP | BBa_K2387054 | BBa_K2387055 |
eYFP | BBa_K2387003 | BBa_K2387065 |
mVenus | BBa_K2387045 | BBa_K2387046 |
sfGFP | BBa_K2387047 | BBa_K2387048 |
mCerulean | BBa_K2387052 | BBa_K2387053 |
//cds/reporter/rfp
//chassis/prokaryote
//function/reporter/fluorescence
abs | Max. 515 nm |
chassis | E. coli |
color | Red Fluorescence, Bright Pink cells |
emission | Max. 607 nm |