Part:BBa_K2387045

mVenus controlled by inducible araC/pBAD promoter

mVenus is a bright yellow monomeric fluorescent protein. In this composite, its expression is regulated by the araC/pBad promoter and by a strong RBS. mVenus presents a maximum pick of excitation at 515 nm and a maximum pick of emission at 528. mVenus was engineered from YFP to grant it a faster maturation and to make more resistant to pH and chloride ions.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 1205
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 1144
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 979
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI site found at 961
  

Functional Parameters

abs	Max. 515 nm
chassis	E. coli
color	Yellow Fluorescence, Pale Yellow cells
emission	Max. 528 nm

In order to test the functionality of this protein under the araC/pBad promoter, the absorbance and the fluorescence spectra were measured after induction with 0.002% arabinose (Figure 1).

Figure 1: Absorbance and fluorescence spectra from mVenus measured after induction with 0.002% arabinose.

This protein is part of a collection of split proteins developed for BiFC analysis and tested with the CpxR system ([[BBa_K2387032]]). These proteins were characterized through multiple experiments using as interacting proteins antiparallel synthetic leucine zippers. The proteins of this collection are the ones found in Table 1.

Table 1: Split Proteins.
Protein	Part Number (Full length)	Part Number (Split Protein)
mRFP	[[BBa_K2387054]]	[[BBa_K2387055]]
eYFP	[[BBa_K2387003]]	[[BBa_K2387065]]
mVenus	[[BBa_K2387045]]	[[BBa_K2387046]]
sfGFP	[[BBa_K2387047]]	[[BBa_K2387048]]
mCerulean	[[BBa_K2387052]]	[[BBa_K2387053]]