Composite

Part:BBa_K2404020

Designed by: Ryan Coates   Group: iGEM17_Cardiff_Wales   (2017-10-16)
Revision as of 10:55, 31 October 2017 by Gparry75 (Talk | contribs)


TSH antagonist under control of the PR2 promoter


This construct contains the PR2 promotor, and the TSHH coding sequence. This does not contain a terminator sequence.
This part is contained within the Phytobrick level 1 pGB-A2 plasmid.
The PR2 promoter is induced by the plant hormone salicylic acid.


Sequence and Features
1-6: BsmBI
16-516: PR2
517-1329: TSHH
1334-1339: BsmBI



Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1045
    Illegal PstI site found at 1087
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 242
    Illegal PstI site found at 1045
    Illegal PstI site found at 1087
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1300
    Illegal XhoI site found at 1112
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1045
    Illegal PstI site found at 1087
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1045
    Illegal PstI site found at 1087
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None