Translational_Unit

Part:BBa_K2505000

Designed by: Hazuki Hasegawa   Group: iGEM17_TokyoTech   (2017-10-16)
Revision as of 10:01, 31 October 2017 by TakumaY (Talk | contribs)

rbs-ahk4

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 76
    Illegal BamHI site found at 1157
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 324
    Illegal SapI.rc site found at 1959

This gene is derived from Arabidopsis thaliana and encode a receptor of cytokinins, AHK4. Cytokinins are signaling molecules (Phytohormones) in plants and play important roles in cell growth and differentiation. AHK4 has a histidine kinase activity, and binding of a cytokinin to AHK4 triggers auto-phosphorylation of AHK4 and the following histidine-to-aspartate phosphorelay. As a consequence, transcription from target genes is induced and/or repressed so that physiological states of plants are changed. The DNA sequences of this gene is optimized for expressing in E. coli cells considering the codon usage.


Characterization

We tested the function of AHK4 protein that receives iP and induces histidine kinase phosphorylation in E.coli cell. Our objective was to characterize the function of AHK4 protein.

Materials & methods

Result

[edit]
Categories
Parameters
None