Composite

Part:BBa_K2311000

Designed by: Wenwen Lin   Group: iGEM17_SiCAU-China   (2017-09-30)
Revision as of 07:42, 31 October 2017 by Lww (Talk | contribs)


Positive feedback loop (LuxI with LVA)

This part has a positive feedback loop, so the expression of GFP can enhance quickly. But because of the LVA tag and LuxR's bug [http://2017.igem.org/Team:SiCAU-China/Contribution (more details about LuxR's bug)], this system could not work unless adding AHL (more than 10^-9M, in BL21).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2315


[edit]
Categories
Parameters
None