DNA
TRXzDP

Part:BBa_K2377004

Designed by: Zhicheng Lin, Ren Yi, He jindian   Group: iGEM17_Lanzhou   (2017-10-23)
Revision as of 07:02, 31 October 2017 by Lexie (Talk | contribs)


TrxzDP, Thioredoxin Z with two reverse T7 promoters and double terminators to generate dsRNA

This one is initial part in our project. It is made up of Thioredoxin z (TRXz) gene fragment with two reverse T7 promoters and double terminators. Lanzhou_Parts_3.png

TRX z is a branch of plastidial thioredoxin (TRX), lying between x- and y-type TRXs thus was named TRX z, which is a likely candidate for mediating thiol-modifications in chloroplasts. Recently, several independent studies could demonstrate that TRX z constitutes a subunit of the plastid-encoded RNA polymerase (PEP). Meanwhile the generation of the full PEP complement is an essential early step during chloroplast bio-genesis and its disturbance cannot be reversed if a certain time point in the program has been passed. The Arabidopsis knockout mutant lines of TRX z exhibit a complete albino phenotype and was inhibited in chloroplast development. Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 284
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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