Composite
sgR 23A

Part:BBa_K2371004

Designed by: Qi Xiao   Group: iGEM17_BGIC-Union   (2017-10-23)
Revision as of 07:01, 31 October 2017 by Mat2017 (Talk | contribs)


sgRNA generator for EML4-ALK variant A 23 This specific sgRNA generator is for detection of EML4-ALK cancer gene detection in pair with BGIC-Union NT7-dCas9(BBa_K2371000) and CT7-dCas9(BBa_K237101). This part is designed with T7 promotor and T7 terminator and can effectively generate specific RNA in vitro. The product can have RNA concentration of 1500 ng/ul or higher with MEGAscript™ T7 Transcription Kit and after the purification and collection using Ambion® mirVana™ miRNA Isolation Kit. BGIC-Union Part sgRNA Figure1 plasmid.png

Figure 1. The plasmid of sgRNA generator. The sgRNA generator Biobrick is composed of a T7 promotor, coding sequence for sgRNA and a T7 terminator


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//function/crispr/cas9
Parameters
function
switch_point