Regulatory

Part:BBa_K2295003

Designed by: Julius Holzschuh   Group: iGEM17_Freiburg   (2017-10-20)
Revision as of 03:31, 31 October 2017 by JuliusH (Talk | contribs)


hre (hypoxia response element) ptal (minimalpromoter)


Chooooooooooooooooo werte

Figure 1: Flow cytometry of hypoxia response element promoter tests.

a) Jurkat, b) HEK293T cells stably transduced with 4xHRE-pTal:eCFP, and c) HRE-pTal:eCFP or d) 4xHRE-pTal:eCFP PEI transfected into CHO-K1 cells. For analysis cells were incubated 24 h with indicated concentrations of CoCl2. Significant differences were determined using one-tailed student’s t-test (Excel 2017) followed by Bonferroni-Hoch correction; * p ** p non-significant and decreasing differences are not marked.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 51
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References

https://parts.igem.org/Part:BBa_K1456004

[edit]
Categories
//classic/regulatory/other
//function/immunology
//promoter
Parameters
None