![](https://parts.igem.org/images/partbypart/icon_regulatory.png)
Regulatory
Part:BBa_K2295003
Designed by: Julius Holzschuh Group: iGEM17_Freiburg (2017-10-20)
hre (hypoxia response element) ptal (minimalpromoter)
Chooooooooooooooooo werte
![](/wiki/images/f/f8/T-FREIBURG-HIF1A_shRNA2_134-50pc.png)
Figure 1: Flow cytometry of hypoxia response element promoter tests.
a) Jurkat, b) HEK293T cells stably transduced with 4xHRE-pTal:eCFP, and c) HRE-pTal:eCFP or d) 4xHRE-pTal:eCFP PEI transfected into CHO-K1 cells. For analysis cells were incubated 24 h with indicated concentrations of CoCl2. Significant differences were determined using one-tailed student’s t-test (Excel 2017) followed by Bonferroni-Hoch correction; * p ** p non-significant and decreasing differences are not marked.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 51
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
References
https://parts.igem.org/Part:BBa_K1456004
[edit]
Categories
Parameters
//classic/regulatory/other
//function/immunology
//promoter
//function/immunology
//promoter
None |