Signalling

Part:BBa_K2295002

Designed by: Julius Holzschuh   Group: iGEM17_Freiburg   (2017-10-20)
Revision as of 03:11, 31 October 2017 by JuliusH (Talk | contribs)


HIF1A (Hypoxia-inducible factor one alpha)

HIF1A

Overview


Western Blot against HIF1A

Figure 7: Transient introduction of artificial HIF1A in HIF1A shRNA2 knockdown HEK293T cell line.

Induction of the overexpressed HIF1A-HA can be observed in the last lane. Endogenous and artificial HIF1A were detected with HIF1A antibody; reintroduced HIF1A was detected via an HA-tag; GAPDH served as a loading control. Wild type (WT) and knockdown (HIF1A shRNA2) cells were transfected with CMV:HIF1A-HA one day prior to induction with CoCl2 to simulate hypoxia. Cells were harvested 24 hours after induction. Immunoblot assay was performed using antibodies against HIF1A, HA-tag and GAPDH.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 79
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 10
    Illegal AgeI site found at 1086
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None