![](https://parts.igem.org/images/partbypart/icon_signalling.png)
Signalling
Part:BBa_K2295002
Designed by: Julius Holzschuh Group: iGEM17_Freiburg (2017-10-20)
HIF1A (Hypoxia-inducible factor one alpha)
Hif1a
![](/wiki/images/f/f8/T-FREIBURG-HIF1A_shRNA2_134-50pc.png)
Figure 1:
Figure 7: Transient introduction of artificial HIF1A in HIF1A shRNA2 knockdown HEK293T cell line.
Induction of the overexpressed HIF1A-HA can be observed in the last lane. Endogenous and artificial HIF1A were detected with HIF1A antibody; reintroduced HIF1A was detected via an HA-tag; GAPDH served as a loading control. Wild type (WT) and knockdown (HIF1A shRNA2) cells were transfected with CMV:HIF1A-HA one day prior to induction with CoCl2 to simulate hypoxia. Cells were harvested 24 hours after induction. Immunoblot assay was performed using antibodies against HIF1A, HA-tag and GAPDH.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 79
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 10
Illegal AgeI site found at 1086 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |